Papers for journal club

The following papers illustrate the usefulness of the analog-sensitive kinase method. The first paper documents the use of analog-sensitive yeast strains to allow specific inhibition of protein kinases in the cell; the second paper illustrates the use of these mutants to identify Cdk1 substrates, as described in the lecture.

Bishop, A.C., Ubersax, J.A., Petsch, D.T., Matheos, D., Gray, N.S., Blethrow, J., Shimizu, E., Tsien, J.Z., Schultz, P.G., Rose, M.D., Wood, J.L., Morgan, D.O., and Shokat, K.M. (2000) A chemical switch for inhibitor-sensitive alleles of any protein kinase. Nature 407, 395-401.

Ubersax, J.A., Woodbury, E.L., Quang, P.N., Paraz, M., Blethrow, J.D., Shah, K., Shokat, K.M., and Morgan, D.O. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425, 859-864.


The following papers illustrate the importance of cyclin specificity in Cdk function. The first paper provides genetic evidence in yeast that the S-phase cyclin Clb5 has greater S-phase promoting activity than the mitotic cyclin Clb2. The second paper includes the identification of Clb5-specific Cdk1 substrates as described in the lecture.

Cross, F.R., Yuste-Rojas, M., Gray, S., and Jacobson, M.D. (1999). Specialization and targeting of B-type cyclins. Mol. Cell 4, 11-19.

Loog, M., and Morgan, D.O. (2005) Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates. Nature 434, 104-108.


The following paper describes the mass spectrometric approach to the identification of Cdk1 substrates, plus discussions of general issues in the function and evolution of phosphorylation sites.

Holt, L.J., Tuch, B.B., Villén, J., Johnson, A.D., Gygi, S.P., and Morgan, D.O. (2009) Global analysis of Cdk1 phosphorylation sites provides insights into evolution. Science 325, 1682-1686.

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