Facilitator Questions

What types of experiments were done to investigate the entry of T. cruzi into cells?  What were the conclusions?

What are the effects of increases in cytosolic calcium concentrations on lysosomes?  What other cellular processes can increases in cytosolic calcium affect?

What did the Andrews lab use Total Internal Reflection Microscopy (TIRFM) to show?


What is the molecular machinery involved in controlling lysosomal exocytosis?  How was this shown?

The Andrew’s lab use Leishmania to study intracellular pathogen survival.  How can this work be related to the study of T. cruzi?

What are the functions of Nramp1 and Nramp2?  Why are they important?

How does Leishmania acquire iron in lysosomal compartments?  How did Dr. Chau Huynh show this?

What are some of the major questions that need to be addressed next?

How (and in what cells type) does Leishmania persist in host tissues? 
How does Leishmania evade the host immune system? 
What are the requirements for Leishmania survival with in the host?

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